Lavacchi D.; Gelmini S.; Calabri A.; Rossi G.; Simi L.; Caliman E.; Mancini I.; Salvianti F.; Petroni G.; Guidolin A.; Scolari F.; Messerini L.; Pillozzi S.; Pinzani P.; Antonuzzo L. (2023). Early changes in circulating tumor DNA (ctDNA) predict treatment response in metastatic KRAS-mutated colorectal cancer (mCRC) patients. HELIYON, vol. 9, pp. 0-0, ISSN:2405-8440 DOI  

  Mancini I.; Simi L.; Salvianti F.; Castiglione F.; Sonnati G.; Pinzani P. (2019). Analytical evaluation of an NGS testing method for routine molecular diagnostics on melanoma formalin-fixed, paraffin-embedded tumor-derived DNA. DIAGNOSTICS, vol. 9, pp. 117-131, ISSN:2075-4418 DOI   Accesso ONLINE all'editore

  Mini E.; Lapucci A.; Perrone G.; D'Aurizio R.; Napoli C.; Brugia M.; Landini I.; Tassi R.; Picariello L.; Simi L.; Mancini I.; Messerini L.; Magi A.; Pinzani P.; Mazzei T.; Tonelli F.; Nobili S. (2019). RNA sequencing reveals PNN and KCNQ1OT1 as predictive biomarkers of clinical outcome in stage III colorectal cancer patients treated with adjuvant chemotherapy. INTERNATIONAL JOURNAL OF CANCER, pp. 0-0, ISSN:0020-7136, Wiley-Liss Inc.: DOI   Accesso ONLINE all'editore

  Righi, Alberto; Mancini, Irene; Gambarotti, Marco; Picci, Piero; Gamberi, Gabriella; Marraccini, Cristina; Dei Tos, Angelo Paolo; Simi, Lisa; Pinzani, Pamela; Franchi, Alessandro* (2017). Histone 3.3 mutations in giant cell tumor and giant cell-rich sarcomas of bone.. HUMAN PATHOLOGY, vol. 68, pp. 128-135, ISSN:0046-8177 DOI   Accesso ONLINE all'editore

  Girolami, Ilaria; Mancini, Irene; Simoni, Antonella; Baldi, Giacomo Giulio; Simi, Lisa; Campanacci, Domenico; Beltrami, Giovanni; Scoccianti, Guido; D'Arienzo, Antonio; Capanna, Rodolfo; Franchi, Alessandro (2016). Denosumab treated giant cell tumour of bone: A morphological, immunohistochemical and molecular analysis of a series. JOURNAL OF CLINICAL PATHOLOGY, vol. 69, pp. 240-247, ISSN:0021-9746 DOI   Accesso ONLINE all'editore

  De Luca, Francesca; Rotunno, Giada; Salvianti, Francesca; Galardi, Francesca; Pestrin, Marta; Gabellini, Stefano; Simi, Lisa; Mancini, Irene; Vannucchi, Alessandro Maria; Pazzagli, Mario; Di Leo, Angelo; Pinzani, Pamela (2016). Mutational analysis of single circulating tumor cells by next generation sequencing in metastatic breast cancer. ONCOTARGET, vol. 7, pp. 26107-26119, ISSN:1949-2553 DOI   Accesso ONLINE all'editore

  Tassi, R; Perrone, G; Brugia, M; Simi, L; Petreni, P; Mori, E; Landini, I; Pazzagli, M; Mazzei, T; Mini, E; Nobili, S (2015). Fluoropyrimidine-related toxicity in gastrointestinal cancer patients. Assessment of risk factors.. ANNALS OF ONCOLOGY, vol. 26, pp. 43-44, ISSN:0923-7534 DOI  

  D Massi; L Simi; E Sensi; G Baroni; G Xue; C Scatena; A Caldarella; P Pinzani; G Fontanini; A Carobbio; C Urso; M Mandalà. (2015). Immunohistochemistry is highly sensitive and specific for the detection of NRASQ61R mutation in melanoma.. MODERN PATHOLOGY, vol. 28, pp. 487-497, ISSN:0893-3952

  Mancini, Irene; Pinzani, Pamela; Simi, Lisa; Brandslund, Ivan; Vermeersch, Pieter; Di Resta, Chiara; Schwab, Matthias; Marc, Janja; van Schaik, Ron; Pazzagli, Mario (2015). Implementation of a companion diagnostic in the clinical laboratory: The BRAF example in melanoma. CLINICA CHIMICA ACTA, vol. 439, pp. 128-136, ISSN:0009-8981 DOI   Accesso ONLINE all'editore

  R Santi; L Simi; R Fucci; M Paglierani; M Pepi; P Pinzani; B Merelli; M Santucci; G Botti; C Urso; D Massi. (2015). KIT genetic alterations in anorectal melanomas.. JOURNAL OF CLINICAL PATHOLOGY, vol. 68, pp. 130-134, ISSN:0021-9746

  Malentacchi, F; Pazzagli, M; Simi, L; Orlando, C; Wyrich, R; Günther, K; Verderio, P; Pizzamiglio, S; Ciniselli, Cm; Zhang, H; Korenková, V; Rainen, L; Bar, T; Kubista, M; Gelmini, S (2014). SPIDIA-RNA: second external quality assessment for the pre-analytical phase of blood samples used for RNA based analyses.. PLOS ONE, vol. 9, pp. 112293-112293, ISSN:1932-6203 DOI  

  Massi D; Pinzani P; Simi L; Salvianti F; De Giorgi V; Pizzichetta MA; Mirri F; Steffan A; Orlando C; Santucci M; Canzonieri V. (2013). BRAF and KIT somatic mutations are present in amelanotic melanoma.. MELANOMA RESEARCH, pp. ---, ISSN:0960-8931

  M. Pazzagli;F. Malentacchi;L. Simi;C. Orlando;R. Wyrich;K. Günther;C. C. Hartmann;P. Verderio;S. Pizzamiglio;C. M. Ciniselli;A. Tichopad;M. Kubista;S. Gelmini (2013). SPIDIA-RNA: First external quality assessment for the pre-analytical phase of blood samples used for RNA based analyses.. METHODS, vol. ...., pp. 20-31, ISSN:1046-2023 DOI   Accesso ONLINE all'editore

  Francesca Malentacchi ;Mario Pazzagli ; Lisa Simi ;Claudio Orlando ; Ralf Wyrich ; Christina Hartmann ; Paolo Verderio ; Sara Pizzamiglio ; Chiara Maura Ciniselli; Alex Tichopad ;Michael Kubista; Stefania Gelmini (2013). SPIDIA-DNA: an External Quality Assessment for the pre-analytical phase of blood samples used for DNA-based analyses.. CLINICA CHIMICA ACTA, vol. 424, pp. 274-286, ISSN:0009-8981 DOI  

  Arvia R; Corcioli F; Simi L; Orlando C; De Santis R; Facchini M; Donatelli I; Azzi A. (2012). Monitoring the susceptibility to oseltamivir of Influenza A(H1N1) 2009 virus by nested-PCR and pyrosequencing during the pandemic and in the season 2010-2011.. JOURNAL OF VIROLOGICAL METHODS, vol. 184, pp. 113-116, ISSN:0166-0934

  Nicola Pratesi; Monica Mangoni; Irene Mancini; Fabiola Paiar; Lisa Simi; Lorenzo Livi; Sara Cassani; Michela Buglione; Salvatore Grisanti; Camillo Almici; Caterina Polli; Calogero Saieva; Stefano Maria Magrini; Giampaolo Biti; Mario Pazzagli; Claudio Orlando (2011). Association between single nucleotide polymorphisms in the XRCC1 and RAD51 genes and clinical radiosensitivity in head and neck cancer.. RADIOTHERAPY AND ONCOLOGY, vol. 99, pp. 356-361, ISSN:0167-8140 DOI   Accesso ONLINE all'editore

  Mazzoni F;Rotella V;Pratesi N;Boni L;Simi L;Orlando C;Comin CE;Maddau C;Di Costanzo F (2011). From clinical trials to clinical practice: predictors of response to erlotinib in advanced non-small cell lung cancer patients pretreated with chemotherapy.. TUMORI, vol. 97, pp. 0-0, ISSN:0300-8916 DOI  

  Massi D; Cesinaro AM; Tomasini C; Paglierani M; Bettelli S; Dal Maso L; Simi L; Salvianti F; Pinzani P; Orlando C; De Giorgi V; Lukic S; Maiorana A; Santucci M; Canzonieri V. (2011). Atypical Spitzoid melanocytic tumors: a morphological,mutational, and FISH analysis. JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY, vol. 64, pp. 919-935, ISSN:0190-9622 DOI  

  Pinzani P; Santucci C; Mancini I; Simi L; Salvianti F; Pratesi N; Massi D; De Giorgi V; Pazzagli M; Orlando C (2011). BRAFV600E detection in melanoma is highlyimproved by COLD-PCR.. CLINICA CHIMICA ACTA, vol. 412, pp. 901-905, ISSN:0009-8981

  Vinci, Serena; Gelmini, Stefania; Pratesi, Nicola; Conti, Simona; Malentacchi, Francesca; Simi, Lisa; Pazzagli, Mario; Orlando, Claudio (2011). Genetic variants in miR-146a, miR-149, miR-196a2, miR-499 and their influence on relative expression in lung cancers. CLINICAL CHEMISTRY AND LABORATORY MEDICINE, vol. 49, pp. 2073-2080, ISSN:1437-4331 DOI  

  D. Massi; A.M. Cesinaro; C. Tomasini; M. Paglierani; S. Bettelli; L. Dal Maso; L. Simi; F. Salvianti; P. Pinzani; C. Orlando; V. De Giorgi; S. Lukic; A. Maiorana; M. Santucci; V. Canzonieri. (2011). Atypical Spitzoid melanocytic tumors: a morphological, mutational, and FISH analysis.. JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY, vol. 64(5), pp. 919-935, ISSN:0190-9622

  Mazzoni F; Rotella V; Pratesi N; Boni L; Simi L; Orlando C; Comin CE; Maddau C; Di Costanzo F (2011). From clinical trials to clinical practice: predictors ofresponse to erlotinib in advanced non-small cell lung cancer patients pretreated with chemotherapy. TUMORI, vol. 97, pp. 160-165, ISSN:0300-8916

  Simi L; Pinzani P; Salvianti F; Lorenzoni A; Tomasini C; Broganelli P; Orlando C; Pazzagli M; Massi D. (2011). Two novel H-RAS mutations identified in a child with anatypical spitzoid tumor.. ARCHIVES OF DERMATOLOGY, vol. 147, pp. 514-515, ISSN:0003-987X

  Vinci S; Gelmini S; Pratesi N; Conti S; Malentacchi F; Simi L; Pazzagli M; Orlando C. (2011). Genetic variants in miR-146a, miR-149, miR-196a2, miR-499 and their influence on relative expression in lung cancers.. CLINICAL CHEMISTRY AND LABORATORY MEDICINE, vol. PubMed PMID: 21902575, pp. ...-..., ISSN:1434-6621

  Simi L; Malentacchi F; Luciani P; Gelmini S; Deledda C; Arvia R; Mannelli M; Peri A; Orlando C (2010). Seladin-1 expression is regulated by promoter methylation inadrenal cancer. BMC CANCER, vol. 10, pp. 201-....., ISSN:1471-2407

  Mancini I; Santucci C; Sestini R; Simi L; Pratesi N; Cianchi F; Valanzano R; Pinzani P; Orlando C. (2010). The use of COLD-PCR and high-resolution melting analysis improbe the limito f detection of KRAS and BRAF mutations in colorectal cancer. JOURNAL OF MOLECULAR BIOLOGY, vol. 12, pp. 705-711, ISSN:1089-8638

  I.Mancini;C.Santucci;R.Sestini;L.Simi;N.Pratesi;F.Cianchi;R.Valanzano;P.Pinzani;C.Orlando (2010). The use of COLD-PCR and High Resolution Melting Analysis improves the limit of detection of KRAS and BRAF mutations in colorectal cancer. THE JOURNAL OF MOLECULAR DIAGNOSTICS, vol. 12, pp. 705-711, ISSN:1525-1578

  Francesca Malentacchi; Lisa Simi; Caterina Nannelli; Matteo Andreani; Alberto Janni; Silvia Pastorekova; Claudio Orlando (2009). Alternative splicing variants of carbonic anhydrase IX in human non-small cell lung cancer.. LUNG CANCER, vol. 64, pp. 271-276, ISSN:0169-5002

  Nicola Fiotti; Nicola Altamura; Claudio Orlando; Lisa Simi; Bernhard Reimers; Pietro Pascotto; Bartolo Zingone; Andrea Pascotto; Mario Serio; Gianfranco Guarnieri; Carlo Giansante (2008). Metalloproteinases-2, -9 and TIMP-1 expression in stable and unstable coronary plaques undergoing PCI.. INTERNATIONAL JOURNAL OF CARDIOLOGY, vol. 127, pp. 350-357, ISSN:0167-5273

  T.Ercolino; L.Becherini; A.Valeri; M.Maiello; M.Gaglianò; G.Parenti; M.Ramazzotti; E.Piscitelli; L.Simi; P.Pinzani; G.Nesi; D.Degl'Innocenti; N.Console; C.Bergamini; M.Mannelli (2008). Uncommon clinical presentations of pheochromocytoma and paraganglioma in two different patients affected by two distinct novel VHL germline mutations. CLINICAL ENDOCRINOLOGY, vol. 68, pp. 762-768, ISSN:0300-0664 DOI   Accesso ONLINE all'editore

  Pratesi N, Simi L, Sestini R, Nobili S, Vignoli M, Landini I, Cianchi F, Valanzano R, Genuardi M, Mini E, Orlando C (2008). The clinical role of PIK3CA mutations in colorectal cancer. In: International Symposium on Cancer genotypes and Cancer phenotypes, Florence, Italy, Luglio 4-5, 2008, -, pp. 69-69.

  C. Filoni; A. Caciotti; L. Carraresi; M.A. Donati; R. Mignani; R. Parini; M. Filocamo; F. Soliani; L. Simi; R. Guerrini; E. Zammarchi; A. Morrone (2008). Unbalanced GLA mRNAs ratio quantified by real-time PCR in Fabry patients' fibroblasts results in Fabry disease.. EUROPEAN JOURNAL OF HUMAN GENETICS, vol. 16(11), pp. 1311-1317, ISSN:1018-4813 DOI  

  Lisa Simi; Nicola Pratesi; Marina Vignoli; Roberta Sestini; Fabio Cianchi; Rosa Valanzano; Stefania Nobili; Enrico Mini; Mario Pazzagli; Claudio Orlando (2008). High-resolution melting analysis for rapid detection of KRAS, BRAF, and PIK3CA gene mutations in colorectal cancer.. AMERICAN JOURNAL OF CLINICAL PATHOLOGY, vol. 130, pp. 247-253, ISSN:0002-9173 DOI  

  Lisa Simi; Pamela Pinzani; Claudia Casini Raggi; Mario Pazzagli; Claudio Orlando (2007). Influence of 17q gain and promoter polymorphisms on mRNA expression of somatostatin receptor type 2 in neuroblastoma.. CLINICA CHIMICA ACTA, vol. 384, pp. 149-154, ISSN:0009-8981 DOI  

  Caciotti A; Donati MA; Procopio E; Filocamo M; Kleijer W; Wuyts W; Blaumeiser B; d'Azzo A; Simi L; Orlando C; McKenzie F; Fiumara A; Zammarchi E; Morrone A. (2007). GM1 gangliosidosis: molecular analysis of nine patients and development of an RT-PCR assay for GLB1 gene expression profiling.. HUMAN MUTATION, vol. 28, pp. 204-204, ISSN:1059-7794 DOI  

  MANNELLI M; ERCOLINO T; GIACHE' V; SIMI L; CIRAMI C; PARENTI G (2007). Genetic screening for pheochromocytoma: should SDHC gene analysis be included?. JOURNAL OF MEDICAL GENETICS, vol. 44, pp. 586-587, ISSN:0022-2593, BMJ / British Medical Journal Publishing Group:PO Box 299, London WC1H 9TD United Kingdom:011 44 20 73836270, EMAIL: subscriptions@bmjgroup.com, INTERNET: http://www.bmjpg.com/bmj, Fax: 011 44 20 73836402:

  MANNELLI M; SIMI L; GAGLIANO' MS; OPOCHER G; ERCOLINO T; BECHERINI L; PARENTI G (2007). Genetics and biology of pheochromocytoma.. EXPERIMENTAL AND CLINICAL ENDOCRINOLOGY & DIABETES, vol. 115, pp. 160-165, ISSN:0947-7349, MVS Medizinverlage Stuttgart:Postfach 301120, D 70451 Stuttgart Germany:011 49 711 8931321, EMAIL: aboservice@thieme.de, Fax: 011 49 711 8931422 Stuttgart; New York: Barth in Georg-Thieme-Verl..:

  R. RECENTI; G. LEONE; L. SIMI; M. ORFEI; P. PINZANI; G. PIERACCINI; G. MONETI; CAROSSINO A.M.; A. FRANCHI; BARTOLUCCI G; CARBONELL SALA S; GINANNESCHI M. ; TANINI A.; BRANDI M.L (2007). Clodronate acts on human osteoclastic cell proliferation, differentiation and function in a bioreversible manner. CLINICAL CASES IN MINERAL AND BONE METABOLISM, vol. 4, pp. 146-155, ISSN:1724-8914

  Lisa Simi; Giulia Venturini; Francesca Malentacchi; Stefania Gelmini; Matteo Andreani; Alberto Janni; Silvia Pastorekova; Claudiu T Supuran; Mario Pazzagli; Claudio Orlando (2006). Quantitative analysis of carbonic anhydrase IX mRNA in human non-small cell lung cancer.. LUNG CANCER, vol. 52, pp. 59-66, ISSN:0169-5002

  Pamela Pinzani; Benedetta Salvadori; Lisa Simi; Simonetta Bianchi; Vito Distante; Luigi Cataliotti; Mario Pazzagli; Claudio Orlando (2006). Isolation by size of epithelial tumor cells in peripheral blood of patients with breast cancer: correlation with real-time reverse transcriptase-polymerase chain reaction results and feasibility of molecular analysis by laser microdissection.. HUMAN PATHOLOGY, vol. 37, pp. 711-718, ISSN:0046-8177

  Mannelli M; Simi L; Ercolino T; Gaglianò MS; Becherini L; Vinci S; Sestini R; Gensini F; Pinzani P; Mascalchi M; Guerrini L; Pratesi C; Nesi G; Torti F; Cipollini F; Bernini GP; Genuardi M. (2006). SDH mutations in patients affected by paraganglioma syndromes: a personal experience.. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, pp. 183-189, ISSN:0077-8923

  CELLAI I; BENVENUTI S; LUCIANI P; GALLI A; CENI E; SIMI L; BAGLIONI S; MURATORI M; OTTANELLI B; M. SERIO; THIELE CJ; PERI A (2006). Antineoplastic effects of rosiglitazone and PPARgamma transactivation in neuroblastoma cells. BRITISH JOURNAL OF CANCER, vol. 95, pp. 879-888, ISSN:0007-0920, Editore attuale:NATURE PUBLISHING GROUP, MACMILLAN BUILDING, 4 CRINAN ST, LONDON, ENGLAND, N1 9XW Harcourt Publishers Limited:Foots Cray High Street, Sidcup Kent DA14 5HP United Kingdom:011 44 20 83085700, EMAIL: journals@harcourt.com, INTERNET: http://www.harcourt-international.com, Fax: 011 44 20 83085876:

  L. SIMI; R. SESTINI; P. FERRUZZI; M. GAGLIANO'; F. GENSINI; M. MASCALCHI; L. GUERRINI; C. PRATESI; P. PINZANI; G. NESI; T. ERCOLINO; M. GENUARDI; M. MANNELLI (2005). Phenotype variability of neural crest-derived tumours in six Italian families segregating the same founder SDHD mutation Q109X.. JOURNAL OF MEDICAL GENETICS, vol. 42(8), pp. 0-0, ISSN:0022-2593 DOI  

  FABIANA ROSATI; IRENE BARDAZZI; PAOLA DE BLASI; LISA SIMI; DINA SCARPI; ANTONIO GUARNA; MARIO SERIO; M. RACCHI; GIOVANNA DANZA (2005). 5alpha-Reductase Activity in Lycopersicon Esculentum: Cloning and Functional Characterization of Ledet2 and Evidences of the Presence of Two Isoenzymes. JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 96, pp. 287-299, ISSN:0960-0760 DOI  

  Raspollini M.R.; Pinzani P.; Simi L.; Amunni G.; Villanucci A.; Paglierani M.; Taddei G.L. (2005). Uterine leiomyosarcomas express KIT protein but lack mutation(s) in exon 9 ofc-KIT.. GYNECOLOGIC ONCOLOGY, vol. 98, pp. 334-335, ISSN:0090-8258

  C CASINI RAGGI; P VERDERIO; M PAZZAGLI; E MARUBINI; L SIMI; P PINZANI; A PARADISO; C. ORLANDO (2005). An Italian Program of External Quality Control for Quantitative Assays Based on Real-Time PCR with Taq-Man Probes.. CLINICAL CHEMISTRY AND LABORATORY MEDICINE, vol. 43, pp. 542-548, ISSN:1434-6621, Walter de Gruyter and Company:Genthinerstrasse 13, D 10785 Berlin Germany:011 49 30 260050, EMAIL: customerservice@deguyter.de, INTERNET: http://www.degruyter.de/, Fax: 011 49 30 26005338: DOI  

  Stefania Gelmini; Melissa Poggesi; Vito Distante; Simonetta Bianchi; Lisa Simi; Michaela Luconi; Claudia Casini Raggi; Luigi Cataliotti; Mario Pazzagli; Claudio Orlando (2004). Tankyrase, a positive regulator of telomere elongation, is over expressed in human breast cancer.. CANCER LETTERS, vol. 216, pp. 81-87, ISSN:0304-3835 DOI  

  CIOPPI F.; SIMI L.; LUCIANI P.; PETRAGLIA F.; SUSINI T.; COBELLIS L.; SERIO M.; M. MAGGI; PERI A. (2004). Expression of uteroglobin and matrix metalloproteinase-9 genes in endometrial cancer: relationship to estrogen and progesterone receptor status.. ONCOLOGY REPORTS, vol. 11, pp. 427-433, ISSN:1021-335X Accesso ONLINE all'editore

  E. SGAMBATI; M. MARINI; G.D. ZAPPOLI THYRION; G. MELLO; C. ORLANDO; L. SIMI; C. TRICARICO; G. GHERI; E. BRIZZI (2004). VEGF expression in the placenta from pregnancies complicated by hypertensive disorders.. BJOG-AN INTERNATIONAL JOURNAL OF OBSTETRICS AND GYNAECOLOGY, vol. 111, pp. 564-570, ISSN:1470-0328

  C. ORLANDO; C. RAGGI C; S. BIANCHI; V. DISTANTE; L. SIMI; V. VEZZOSI; S. GELMINI; P. PINZANI; M. SMITH CAMERON; A. BUONAMANO; E. LAZZERI; M. PAZZAGLI; L. CATALIOTTI; M. MAGGI; M. SERIO (2004). Measurement of somatostatin receptor subtype 2 mRNA in breast cancer and corresponding normal tissue. ENDOCRINE-RELATED CANCER, vol. 11, pp. 323-332, ISSN:1351-0088

  Lisa Simi; Matteo Andreani; Federico Davini; Alberto Janni; Mario Pazzagli; Mario Serio; Claudio Orlando (2004). Simultaneous measurement of MMP9 and TIMP1 mRNA in human non small cell lung cancers by multiplex real time RT-PCR.. LUNG CANCER, vol. 45, pp. 171-179, ISSN:0169-5002

  R. RECENTI; G. LEONE; L. SIMI; M. ORFEI; P. PINZANI; A.M. CAROSSINO; G. PIERACCINI; G. MONETI; A. FRANCHI; M. GINANNESCHI; A. TANINI; M.L. BRANDI (2003). Clodronate modulates osteoclastogenesis by ATP analog, adenosine 5-(b,g-dichloromethylene) triphosphate, in human cell of the osteoclastic lineage with no toxic. ITALIAN JOURNAL OF BIOCHEMISTRY, vol. 52, pp. 107-107, ISSN:0021-2938

  A. Becciolini; S. Porciani; A. Lanini; C. Chadwick; M. Balzi; L. Simi; P. Faraoni; C.S. Potten (1997). Protective effect of TGFß3 on the irradiated small instestine of mice.. In: Convegno, vol. Cell Prolif. vol. 30, pp. 435-435.