Riceve su appuntamento nel suo ufficio presso il Dipartimento di Chimica "Ugo Schiff", in via della Lastruccia 3 a Sesto Fiorentino, secondo piano, stanza 361. Contattare preventivamente il docente tramite email per fissare data e ora.
Enrico Luchinat è nato nel 1985. Si è laureato nel 2007 in Chimica cum laude nel 2009 e in Chimica delle Molecole Biologiche cum laude presso l'Università di Firenze. Nel 2013 ha conseguito il dottorato di ricerca “International Doctorate in Mechanistic and Structural Systems Biology” presso la stessa università, sotto la supervisione della Prof.ssa Lucia Banci. È stato borsista post-dottorato (2013-2019) presso il Dipartimento di Scienze Biochemiche Sperimentali e Cliniche e ricercatore presso il Dipartimento NEUROFARBA (2020-2021). Nel 2022 è entrato a far parte del Dipartimento di Scienze e Tecnologie Agro-alimentari dell'Università di Bologna come RTDa, e nel 2024 è entrato nel Dipartimento di Chimica dell'Università di Firenze come RTDb.
È co-autore più di 45 pubblicazioni peer-reviewed ed è stato invitato come relatore in oltre 30 conferenze internazionali e eventi virtuali. Nel 2020 ha ricevuto il GIDRM Under-35 Award per lo sviluppo di approcci innovativi di NMR in cellula.
Curriculum Vitae
Enrico Luchinat
Identificativi Web
ORCID ID: 0000-0003-4183-4311
Scopus Author ID: 37066145400
WoS ResearcherID: O-1776-2014
Google Scholar ID: 2pEt8dYAAAAJ
Abilitazione Scientifica Nazionale
07/05/2019 – 07/05/2029ASN 2° Fascia – Fondamenti delle Scienze Chimiche e Sistemi Inorganici (03/B1)
10/05/2019 – 10/05/2029ASN 2° Fascia – Biochimica Generale (05/E1)
Esperienza lavorativa
15/12/2023 – OGGI Dipartimento di Chimica “Ugo Schiff”, Università degli studi di Firenze, via della Lastruccia 3, 50019 Sesto Fiorentino, ItaliaRicercatore a Tempo Determinato lettera b (RTDb)02/11/2021 – 14/12/2023
Dipartimento di Scienze e Tecnologie Agro-alimentari – DISTAL, Alma Mater Studiorum – Università di Bologna, Piazza Goidanich 60, 47521 Cesena, ItaliaRicercatore a Tempo Determinato lettera a (RTDa)01/02/2021 – 01/11/2021
Dipartimento NEUROFARBA – Dipartimento di Neuroscienze, Psicologia, Area del Farmaco e Salute del Bambino, Università degli Studi di Firenze, Viale Pieraccini 6, 50139 Firenze, ItaliaBorsa di ricerca: “Sviluppo di metodi NMR utili allo studio delle interazioni che intercorrono tra ligando e proteina finalizzate ad applicazioni biomediche”
03/02/2020 – 31/01/2021 CSGI – Consorzio interuniversitario per lo sviluppo dei Sistemi a Grande Interfase, via della Lastruccia 3, 50019 Sesto Fiorentino (FI), ItaliaBorsa di ricerca: “Sviluppo e applicazione di metodi NMR per l'osservazione di interazioni proteina-legante”
20/01/2019 – 19/01/2020 Dipartimento di Scienze Biomediche Sperimentali e Cliniche “Mario Serio”, Università degli Studi di Firenze, viale Morgagni 50, Firenze (FI), ItaliaBorsa di ricerca: “Effetto del cadmio sul processo di maturazione della superossido dismutasi 1 in cellule umane”
01/01/2018 – 31/12/2018 Dipartimento di Scienze Biomediche Sperimentali e Cliniche “Mario Serio”, Università degli Studi di Firenze, viale Morgagni 50, Firenze (FI), ItaliaAssegno di ricerca: “Ruolo della via di segnalazione di sfingosina 1 fosfato nelle alterazioni metaboliche indotte da cisplatino in progenitori murini della vescicola otica”
01/01/2013 – 31/12/2017 Dipartimento di Scienze Biomediche Sperimentali e Cliniche “Mario Serio”, Università degli Studi di Firenze, viale Morgagni 50, Firenze (FI), ItaliaAssegno di ricerca: “Caratterizzazione di proteine a livello atomico in cellule viventi”
Istruzione e Formazione
01/01/2010 – 31/12/2012. Titolo conseguito il 09/01/2013CERM – Centro di Risonanze Magnetiche, Università degli Studi di FirenzeTitolo della Tesi: “In-cell NMR for structural and functional studies of proteins in their native environment”Dottorato di Ricerca: “International Doctorate in Mechanistic and Structural Systems Biology” (XXV ciclo)Excellent
2007 – 2009. Titolo conseguito il 27/10/2009Università degli Studi di FirenzeTitolo della tesi: “In-cell NMR for structural and functional studies of proteins in their native environment”Laurea Specialistica in Chimica delle Molecole Biologiche (classe 62/S)110/110 cum laude
2004 – 2007. Titolo conseguito il 10/12/2007Università degli Studi di FirenzeTitolo della tesi: “Assegnamento Automatico di Spettri NMR del Backbone di Proteine”Laurea in Chimica (classe 21)110/110 cum laude
Finanziamenti
28/09/2023 – 27/09/2025Ministero dell’Università e della Ricerca (MUR)PRIN 2022, 2022WANFH5 - Time-resolved magnetic resonance to investigate dynamic events in biological systems and biotransformations (TiReD)228.138 €69.400 € (a DISTAL – Università di Bologna)
01/10/2019 – 30/09/2021Instruct-ERIC pan-European research infrastructure in structural biologyJoint Research Award 20.000 €13.000 € (a CERM/CIRMMP Instruct Core Centre Italy)
01/11/2014 – 28/02/2015European Molecular Biology OrganizationEMBO Short-Term Fellowship8.313,10 €
Attività Didattiche
AA 24-25 “Laboratorio di Chimica Inorganica I” (6 CFU), Laurea Magistrale in Chimica, Università di Firenze. Lingua italiana; “Superstructures and Multi-Components Architectures in Life and Material Sciences” (2 CFU), Laurea Magistrale in Advanced Molecular Sciences, Università di Firenze. Lingua inglese.
AA 23-24 “Proteine e Loro Interazioni con Laboratorio” (1 CFU), Laurea Magistrale in Biotecnologie Molecolari, Università di Firenze. Lingua italiana.
AA 22-23 “Chimica Strutturale dei Sistemi Biologici” (4 CFU), Laurea Magistrale in Scienze e Tecnologie Alimentari, DISTAL, Università di Bologna. Lingua italiana.
AA 21-22 “Chimica Strutturale dei Sistemi Biologici” (4 CFU), Laurea Magistrale in Scienze e Tecnologie Alimentari, DISTAL, Università di Bologna. Lingua italiana.
12/2022 “In-cell NMR”, nell’ambito del Corso di Dottorato “International Doctorate in Structural Biology” (2 CFU). Lingua inglese.
06/2019 “In-cell NMR”, nell’ambito del Corso di Dottorato “International Doctorate in Structural Biology” (2 CFU). Lingua inglese.
Attività di Tutoraggio
11/2022 – 04/2023Gabriele PampalonUniversità degli Studi di Firenze“Caratterizzazione di proteine fluorurate in cellule umane tramite spettroscopia 19F in-cell NMR e cristallografia a raggi X”Laurea in Biotecnologie, indirizzo BiomolecolareCorrelatore di Tesi
03/2022 – 10/2022Azzurra CostantinoUniversità degli Studi di Firenze“Studio dell’incorporazione di amminoacidi fluorurati in proteine espresse in cellule umane tramite spettroscopia NMR e cristallografia a raggi X”Laurea Magistrale in Scienze Chimiche, curriculum Molecole BiologicheCorrelatore di Tesi
05/2021 – 01/2022Tommaso BayonUniversità degli Studi di Firenze“Studio di monocolture di cellule HeLa e linfociti T umani tramite spettroscopia NMR in-cellula”Laurea in Biotecnologie, indirizzo BiomolecolareCorrelatore di Tesi
01/2021 – 06/2021Sofia BaldiUniversità degli Studi di Firenze“Studio di interazioni proteina - proteina in cellule umane tramite in-cell NMR”Laurea in Biotecnologie, indirizzo BiomolecolareCorrelatore di Tesi
Organizzazione di Congressi e Workshop
1. iNEXT-Discovery In-cell NMR Training Course, CERM, Sesto Fiorentino, Italy, September 25th – 29th 2023.Organizer
2. Instruct Practical School on Advanced Isotopic Labelling Methods for NMR, IBS – Grenoble, France, September 16th – 23rd 2022.Co-organizer
3. Instruct Training Course: In-cell NMR Analysis of Biomolecular Structure and Function, CERM, Sesto Fiorentino, Italy, December 2nd-6th, 2013.Organizing committee
4. 12th Chianti/Instruct Workshop on BioNMR - Electron and Nuclear Relaxation for Structural Biology, Montecatini Terme, Italy, June 19th-22nd, 2012.Organizing committee
Attività di Peer-Review
Peer Reviewer per le seguenti riviste scientifiche internazionali:
Analytica Chimica Acta, Analytical Chemistry, Angewandte Chemie, Arabian Journal of Chemistry, Biochemical Journal, ChemBioChem, Communications Biology, Data in Brief, International Journal of Molecular Sciences, Journal of Biological Inorganic Chemistry, Journal of Biomolecular NMR, Journal of Enzyme Inhibition and Medicinal Chemistry, Journal of Magnetic Resonance Open, Journal of Medicinal Chemistry, Journal of Physical Chemistry, Journal of the American Chemical Society, Magnetic Resonance in Chemistry, Molecules, Nature Communications, Pharmaceuticals, PNAS Nexus, Proceedings of the National Academy of Sciences of the United States of America, Protein Science, Science Advances, Scientific Reports.
Premi e Riconoscimenti
Under 35 GIDRM Award - Gruppo Italiano Discussione Risonanze Magnetiche, 5° edizione, 2020.
Indici Bibliometrici
h-index: 25 (Scopus), 26 (Google Scholar)Citazioni: 1749 (Scopus), 2321 (Google Scholar)Pubblicazioni: 49Capitoli di libri: 4
Pubblicazioni Scientifiche
1. Nibali, S.C., De Siervi, S., Luchinat, E., Magrì, A., Messina, A., Brocca, L., Mantovani, S., Oliviero, B., Mondelli, M.U., De Pinto, V. Turato, C., Arrigoni, C., Lolicato, M. VDAC1-interacting molecules promote cell death in cancer organoids through mitochondrial-dependent metabolic interference. Iscience, 27(6) (2024). IF: 4.6
2. Alburquerque-González, B., Montoro-García, S., Bernabé-García, Á., Bernabé-García, M., Campioni-Rodrigues, P., Rodríguez-Martínez, A., Luque, I., Salo, T., Pérez-Garrido, A., Pérez-Sánchez, H., Cayuela, M.L. Luengo-Gil, G., Luchinat, E., Postigo-Corrales, F., Staderini, T., Nicolás, F.J., Conesa-Zamora, P. Monastrol suppresses invasion and metastasis in human colorectal cancer cells by targeting fascin independent of kinesin-Eg5 pathway. Biomedicine & Pharmacotherapy, 175, 116785 (2024). IF: 6.9
3. Costantino, A., Pham, L. B., Barbieri, L., Calderone, V., Ben‐Nissan, G., Sharon, M., Banci, L.*, Luchinat, E.*. Controlling the incorporation of fluorinated amino acids in human cells and its structural impact. Protein Science, 33(3), e4910 (2024). IF: 8.0
4. Luchinat, E.*, Barbieri, L., Davis, B., Brough, P. A., Pennestri, M., Banci, L.* Ligand-Based Competition Binding by Real-Time 19F NMR in Human Cells. Journal of Medicinal Chemistry, 67(2), 1115-1126 (2024). IF: 7.3
5. Luchinat, E., & Banci, L. In-cell NMR: Recent progresses and future challenges. Rendiconti Lincei. Scienze Fisiche e Naturali, 34(3), 653-661 (2023). IF: 2.0
6. Pham, L.B.T., Costantino, A., Barbieri, L., Calderone, V., Luchinat, E.*, Banci, L.* Direct Expression of Fluorinated Proteins in Human Cells for 19F In-Cell NMR Spectroscopy. Journal of the American Chemical Society, 145(2), 1389–1399 (2023). IF: 16.383
7. Figiel, M., Szubert, F., Luchinat, E., Bonarek, P., Baranowska, A., Wajda-Nikiel, K., Wilamowski, M., Miłek, P., Dziedzicka-Wasylewska, M., Banci, L. and Górecki, A. Zinc controls operator affinity of human transcription factor YY1 by mediating dimerization via its N-terminal region. Biochimica et Biophysica Acta (BBA)-Gene Regulatory Mechanisms, 1866(1), 194905 (2023). IF: 6.304
8. Theillet, F.-X. & Luchinat, E. In-cell NMR: Why and how? Progress in Nuclear Magnetic Resonance Spectroscopy, 132-133, 1–112 (2022). IF: 9.447
9. Luchinat, E. & Banci, L. In-cell NMR: From target structure and dynamics to drug screening. Current Opinion in Structural Biology, 74, 102374 (2022). IF: 7.786
10. Luchinat, E.*, Cremonini, M., Banci, L.* Radio Signals from Live Cells: The Coming of Age of In-Cell Solution NMR. Chemical Reviews, 122(10), 9267–9306 (2022). IF: 60.620
11. Luchinat, E.*, Barbieri, L., Cremonini, M., Pennestri, M., Nocentini, A., Supuran, C.T., Banci, L.* Determination of intracellular protein–ligand binding affinity by competition binding in-cell NMR. Acta Crystallographica Section D: Structural Biology, 77(10) (2021). IF: 7.652
12. Polykretis, P.*, Luchinat, E.* Biophysical characterization of the interaction between the full-length XIAP and Smac/DIABLO. Biochemical and Biophysical Research Communications, 568, 180-185 (2021). IF: 3.575
13. Barbieri, L., Luchinat, E.* Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor. Journal of Visualized Experiments, 169, e62323 (2021). IF: 1.400
14. Luchinat, E.*, Barbieri, L., Cremonini, M., Banci, L.* Protein in-cell NMR spectroscopy at 1.2 GHz. Journal of Biomolecular NMR, 75(2-3), 97-107 (2021). IF: 2.620
15. Alburquerque-González, B., Bernabé-García, Á., Bernabé-García, M., Ruiz-Sanz, J., López-Calderón, F.F., Gonnelli, L., Banci, L., Peña-García, J., Luque, I., Nicolás, F.J., Cayuela-Fuentes, M.L., Luchinat, E., Pérez-Sánchez, H., Montoro-García, S., Conesa-Zamora, P. The FDA-Approved Antiviral Raltegravir Inhibits Fascin1-Dependent Invasion of Colorectal Tumor Cells In Vitro and In Vivo. Cancers, 13(4), p.861 (2021). IF: 6.639
16. Luchinat, E.*, Barbieri, L., Cremonini, M., Nocentini, A., Supuran, C.T., Banci, L.* Intracellular Binding/Unbinding Kinetics of Approved Drugs to Carbonic Anhydrase II Observed by in-Cell NMR. ACS Chemical Biology, 15(10), 2792–2800 (2020). IF: 4.434
17. Luchinat, E.*, Barbieri, L., Campbell, T.F., Banci, L.* Real-time quantitative in-cell NMR: ligand binding and protein oxidation monitored in human cells using multivariate curve resolution. Analytical Chemistry, 92(14), 9997-10006 (2020). IF: 6.785
18. Luchinat, E., Barbieri, L., Cremonini, M., Nocentini, A., Supuran, C.T., Banci, L. Drug screening in human cells by NMR spectroscopy allows the early assessment of drug potency. Angewandte Chemie Int. Ed., 59(16), 6535-6539 (2020). IF: 12.959
19. Polykretis, P., Luchinat, E., Boscaro, F., Banci, L. Methylglyoxal interaction with superoxide dismutase 1. Redox Biology, 30, 101421 (2020). IF: 9.986
20. Barbieri, L., Luchinat, E.* Backbone resonance assignment of human DJ-1 in the reduced state and in the cysteine sulfinic acid state. Biomolecular NMR assignments, 13(2), 371-376 (2019). IF: 0.576
21. Polykretis, P., Luchinat, E., Bonucci, A., Giachetti, A., Graewert, M. A., Svergun, D. I., Banci, L. Conformational characterization of full-length X-chromosome-linked inhibitor of apoptosis protein (XIAP) through an integrated approach. IUCrJ, 6(5) (2019). IF: 4.756
22. Polykretis, P., Cencetti, F., Donati, C., Luchinat, E.*, Banci, L.* Cadmium effects on superoxide dismutase 1 in human cells revealed by NMR. Redox Biology, 21, 101102 (2019). IF: 7.793
23. Cerofolini, L., Giuntini, S., Barbieri, L., Pennestri, M., Codina, A., Fragai, M., Banci, L., Luchinat, E.*, Ravera, E.* Real-time insights into biological events: in-cell processes and protein-ligand interactions. Biophysical Journal, 116(2), 239-247 (2019). IF: 3.665
24. Luchinat, E., & Banci, L. In-Cell NMR in Human Cells: Direct Protein Expression Allows Structural Studies of Protein Folding and Maturation. Accounts of Chemical Research, 51(6), 1550-1557 (2018). IF: 21.661
25. Capper, M. J.; Wright, G. S. A.; Barbieri, L.; Luchinat, E.; Mercatelli, E.; McAlary, L.; Yerbury, J. J.; O’Neill, P. M.; Antonyuk, S. V.; Banci, L. & Hasnain, S. S. The Cysteine-Reactive Small Molecule Ebselen Facilitates Effective SOD1 Maturation. Nature Communications, 9(1), 1693 (2018). IF: 12.353
26. Luchinat, E., & Banci, L. New structural and functional insights from in-cell NMR. Emerging Topics in Life Sciences, 2(1), 29-38 (2018). IF: n/a
27. Mitri, E., Barbieri, L., Vaccari, L., & Luchinat, E.* 15N isotopic labelling for in-cell protein studies by NMR spectroscopy and single-cell IR Synchrotron Radiation FTIR Microscopy: a correlative study. Analyst, 143, 1171-1181 (2018). IF: 3.864
28. Barbieri, L., Luchinat, E., & Banci, L. Intracellular metal binding and redox behavior of human DJ-1. JBIC Journal of Biological Inorganic Chemistry, 23(1), 61-69 (2018). IF: 2.952
29. Luchinat, E., Chiarella, S., Franceschini, M., Di Matteo, A., Brunori, M., Banci, L., & Federici, L. Identification of a novel nucleophosmin‐interaction motif in the tumor suppressor p14arf. The FEBS journal, 285(5), 832-847 (2018). IF: 4.530
30. Luchinat, E., Barbieri, L., & Banci, L. A molecular chaperone activity of CCS restores the maturation of SOD1 fALS mutants. Scientific reports, 7(1), 17433 (2017). IF: 4.122
31. Hou, M. M., Polykretis, P., Luchinat, E., Wang, X., Chen, S. N., Zuo, H. H., Yang, Y., Chen, J. L., Ye, Y., Li, C., Banci, L. & Su, X. C. Solution structure and interaction with copper in vitro and in living cells of the first BIR domain of XIAP. Scientific reports, 7(1), 16630 (2017). IF: 4.122
32. Luchinat, E., & Banci, L. In-cell NMR: a topical review. IUCrJ, 4(2), 108-118 (2017). IF: 6.544
33. Billè, F., Kourousias, G., Luchinat, E., Kiskinova, M., & Gianoncelli, A. X-ray fluorescence microscopy artefacts in elemental maps of topologically complex samples: Analytical observations, simulation and a map correction method. Spectrochimica Acta Part B: Atomic Spectroscopy, 122, 23-30 (2016). IF: 2.854
34. Barbieri, L., Luchinat, E., & Banci, L. Characterization of proteins by in-cell NMR spectroscopy in cultured mammalian cells. Nature protocols, 11(6), 1101-1111 (2016). IF: 12.423
35. Fuccio, C., Luchinat, E., Barbieri, L., Neri, S., & Fragai, M. Algal autolysate medium to label proteins for NMR in mammalian cells. Journal of biomolecular NMR, 64(4), 275-280 (2016). IF: 2.534
36. Luchinat, E.*, Secci, E., Cencetti, F., & Bruni, P. Sequential protein expression and selective labeling for in-cell NMR in human cells. Biochimica et Biophysica Acta (BBA)-General Subjects, 1860(3), 527-533 (2016). IF: 3.679
37. Luchinat, E., & Banci, L. A unique tool for cellular structural biology: in-cell NMR. Journal of Biological Chemistry, 291(8), 3776-3784 (2016). IF: 4.010
38. Mercatelli, E., Barbieri, L., Luchinat, E., & Banci, L. Direct structural evidence of protein redox regulation obtained by in-cell NMR. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1863(2), 198-204 (2016). IF: 4.651
39. Secci, E., Luchinat, E., & Banci, L. The Casein Kinase 2‐Dependent Phosphorylation of NS5A Domain 3 from Hepatitis C Virus Followed by Time‐Resolved NMR Spectroscopy. ChemBioChem, 17(4), 328-333 (2016). IF: 2.774
40. Barbieri, L., Luchinat, E., Banci, L. Protein interaction patterns in different cellular environments are revealed by in-cell NMR. Scientific Reports 5, 14456 (2015). IF: 4.122
41. Luchinat, E., Gianoncelli, A., Mello, T., Galli, A., Banci, L. Combining in-cell NMR and X-ray fluorescence microscopy to reveal the intracellular maturation states of human superoxide dismutase 1. Chemical Communications 51(3), 584-587 (2015). IF: 6.290
42. Luchinat, E., Barbieri, L., Rubino, J.T., Kozyreva, T., Cantini, F., Banci, L., In-cell NMR reveals potential precursor of toxic species from SOD1 fALS mutants. Nature Communications 5, 5502 (2014). IF: 12.353
43. Barbieri, L., Luchinat, E., Banci, L., Structural insights of proteins in sub-cellular compartments: In-mitochondria NMR. BBA Molecular Cell Research. 1843(11), 2492-6 (2014). IF: 4.651
44. § Banci, L., Barbieri,L., Luchinat,E., Secci,E., Visualization of redox-controlled protein fold in living cells. Chemistry & Biology 20(6), 747-752 (2013). IF: 6.743
45. § Banci,L., Barbieri,L., Bertini,I., Luchinat,E., Secci,E., Zhao,Y., Aricescu,A.R., Atomic-resolution monitoring of protein maturation in live human cells by NMR. Nature Chemical Biology 9(5), 297-9 (2013). IF: 13.843
46. § Banci,L., Barbieri,L., Bertini,I., Cantini,F., Luchinat,E. In-cell NMR in E.coli to monitor maturation steps of hSOD1. Plos ONE 6, e23561 (2011). IF: 2.766
47. § Banci L., Bertini I., Cefaro C., Cenacchi L., Ciofi-Baffoni S., Felli I.C., Gallo A., Gonnelli L., Luchinat E., Sideris D., Tokatlidis K. Molecular chaperone function of Mia40 triggers consecutive induced folding steps of the substrate in mitochondrial protein import. Proc Natl Acad Sci U S A. 107, 20190-5 (2010). IF: 9.504
Libri/Capitoli
1. Bruno, F., Luchinat, E., Kazimierczuk, K., Ravera, E. Fast 2D NMR to Investigate Dynamic Events in Biomolecules. Fast 2D Solution-state NMR: Concepts and Applications, 284-310 (2023).
2. Luchinat, E. & Banci, L., Protein Folding, Maturation and Redox State. In-cell NMR Spectroscopy, 207-227 (2020).
3. Luchinat, E. & Banci, L., In-cell NMR in Eukaryotic Cells–II (Direct Protein Expression). In-cell NMR Spectroscopy, 45-61 (2020).
4. Luchinat, E. NMR spectroscopy: from basic concepts to advanced methods. From Molecules to Living Organisms: An Interplay Between Biology and Physics: Lecture Notes of the Les Houches School of Physics: Volume 102, July 2014, 102, 134 (2016).
Relatore su Invito a Conferenze e Seminari
1. EUROMAR 2024 - The 20th European Magnetic Resonance Congress, Bilbao, Spain, June 30th - July 4th 2024. Invited Speaker. Title: Multi-target ligand binding assay in human cells by real-time in-cell 19F NMR
2. Final iNEXT-Discovery Consortium Meeting and 4th Symposium on Recent Advances in Cryo-EM, Brno, Czech Republic, June 10th-13th 2024. Invited Speaker. Title: Detecting protein-drug interactions in human cells by real-time 19F NMR
3. AILM2024 - Advanced Isotope Labelling Methods for Integrated Structural Biology, Grenoble, France, May 28th –31st 2024.Invited Speaker. Title: Expression of fluorinated proteins in human cells for 19F in-cell NMR
4. iNEXT-Discovery In-cell NMR Training Course, CERM, Sesto Fiorentino, Italy, September 25th – 29th 2023.Organizer / Speaker / Instructor
5. ISMAR 2023 Conference, Brisbane, Australia, August 20th – 25th 2023.Invited Speaker. Title: Real-time monitoring of protein-drug interactions in human cells by protein- and ligand-based 19F NMR
6. 5th Reaction Monitoring Symposium, University of Bath, UK, July 4th 2023.Invited Speaker. Title: Monitoring ligand binding in human cells by real-time in-cell NMR
7. Biophysical Seminar Series, University of Cambridge, UK, June 7th 2023.Invited Speaker. Title: In-cell NMR for real-time studies of protein-ligand interactions in human cells
8. ICGEB course “NMR for combatting diseases: from cancer to SARS-CoV-2”, CERM, Sesto Fiorentino, Italy, March 27th – 31st 2023. Invited Speaker. Title: In-cell NMR for real-time studies of protein-ligand interactions in human cells
9. Instruct Practical School on Advanced Isotopic Labelling Methods for NMR, IBS – Grenoble, France, September 16th – 23rd 2022.Co-organizer / Instructor (lesson + practical). Title: In-cell NMR spectroscopy in human cells
10. EUROMAR 2022 Satellite meeting: Magnetic Resonance at Ultra-High Field, Utrecht, Netherlands, July 15th 2022.Invited Speaker. Title: Solution NMR at 1.2 GHz in vitro and in cells
11. EUROMAR 2022 Conference, Utrecht, Netherlands, July 10th – 14th 2022.Promoted talk. Title: Protein- and ligand-observed 19F NMR spectroscopy in human cells
12. iNEXT-Discovery’s back-to-back JRA and industry workshop, Bad Homburg, Germany, June 7th – 9th 2022.Invited Speaker. Title: Protein-drug interactions monitored by time-resolved NMR in human cells
13. 4th Satellite Meeting on Carbonic Anhydrases, Parma, Italy, November 14th – 17th 2019.Invited Speaker, Poster presentation. Title: Screening of carbonic anhydrase inhibitors directly in human cells by NMR
14. iNEXT Annual Users Meeting 2019, Lund, Sweden, June 26th – 28th 2019.Invited Speaker. Title: Recent developments and applications of in-cell NMR in mammalian cells
15. International Practical School on Advanced Isotopic Labelling Methods for NMR, IBS, Grenoble, France, 29th March – April 5th 2019. Instructor. Title: Practical aspects of in cell NMR spectroscopy in mammalian cells
16. AILM2019 - Advanced Isotopic Labelling Methods for Integrated Structural Biology, IBS, Grenoble, France, March 26th –29th 2019.Invited Speaker. Title: Recent developments and applications of in-cell NMR in mammalian cells
17. CCPN Conference 2018, University of Kent, Canterbury, UK, September 6th – 8th 2018.Invited Speaker. Title: Protein expression in human cells for in-cell NMR
18. Symposium “The Future of Magnetic Resonance”, BMRZ Biocenter University of Frankfurt, Germany, July 18th – 19th 2018.Invited Speaker. Title: Protein expression in human cells for in-cell NMR
19. GIDRM Workshop: Advanced Hardware, Methods and Applications in NMR/MRI, University of L’Aquila, Italy, June 7th – 8th 2018.Invited Speaker. Title: In-cell NMR: how to do it and why?
20. EMBO Workshop “Challenges for magnetic resonance in life sciences”, Grosseto, Italy, May 27th – 31st 2018.Invited Speaker. Title: Rescuing the faulty maturation of SOD1 fALS mutants in human cells: an application of in-cell NMR
21. COST Workshop “Emerging Magnetic Resonance Techniques to Study Dynamic Molecular Machines”, Setúbal, Portugal, April 14th – 16th 2018.Invited Speaker. Title: Protein expression in human cells for in-cell NMR spectroscopy
22. Advanced Isotopic Labelling Methods for Integrated Structural Biology - International Workshop (AILM2017), Grenoble, France, March 6th – 9th 2017.Invited Speaker. Title: Protein expression and selective labelling in mammalian cells for in-cell NMR
23. CCPN/CCPBioSim Joint Conference, Buxton, Derbyshire, UK, July 25th – 28th, 2016.Invited Speaker. Title: Protein folding and maturation in mammalian cells by NMR
24. Les Houches Summer School on Integrated Structural Cell Biology, Les Houches, France, July 2014.Teacher. Title: NMR spectroscopy, from basic concepts to advanced methods (3 lessons)
25. Instruct Training Course: In-cell NMR Analysis of Biomolecular Structure and Function, CERM, Sesto Fiorentino, Italy, December 2nd-6th, 2013.Organizing committee / Tutor / Speaker. Title: Protein expression in mammalian systems / Protein folding and maturation in mammalian cells by NMR
26. GIDRM XLI National Congress on Magnetic Resonance, Pisa, Italy, September 17th-19th, 2012.Selected talk. Title: Monitoring protein maturation steps in living cells with atomic resolution by NMR
27. The 26th Annual Symposium of The Protein Society, San Diego, CA, U.S.A., August 5th-8nd, 2012. Invited speaker. Title: Protein maturation steps monitored with atomic resolution in living cells by NMR
28. 12th Chianti/Instruct Workshop on BioNMR - Electron and Nuclear Relaxation for Structural Biology, Montecatini Terme, Italy, June 19th-22nd, 2012.Organizing committee / Invited speaker / Poster presentation. Title: Monitoring protein maturation steps in living cells with atomic resolution by NMR
Relatore su Invito a Webinar e Conferenze Virtuali
1. Practical Course – In-cell NMR spectroscopy: principles and practice, University of Beira Interior (CICS-UBI), Covilhã, Portugal. Online TeacherTitle: In-cell NMR spectroscopy in human cells: practical aspects and applications (3 hours)June 12th – 16th 2023
2. Seminar – Forschungszentrum Jülich Title: In-cell NMR in human cells: from protein maturation to real-time ligand screening (50’)March 8th 2022
3. Molecular Biosciences PhD program ITQB-Nova, Lisbon, Portugal. Teacher Title: In-cell NMR in human cells: from protein maturation to real-time ligand screening (50’)February 24th 2022
4. TIMB3 Training School - Fundamentals of magnetic resonance spectroscopies and metal trafficking. Lecturer Title: In-cell NMR in human cells: from protein maturation to real-time ligand screening (50’)October 22nd 2021
5. Seminar – Oregon State University Title: In-cell NMR in human cells: from protein maturation to real-time ligand screening (50’)October 6th 2021
6. EUROMAR 2021 Conference. Invited SpeakerTitle: Protein-drug interactions monitored by time-resolved NMR in human cells (20') July 5th – 8th, 2021.
7. Message From The Cell - A workshop on biophysical studies for structural biology inside cells. Invited SpeakerTitle: Protein-drug interactions monitored by time-resolved NMR in human cells (30') June 24th – 25th, 2021.
8. NMR Workshop – Rensselaer Polytechnic InstituteTitle: In-cell NMR in human cells: from protein maturation to real-time ligand screening (60’)May 26th 2021
9. ICMRBS Early Career Researcher Webinar SeriesTitle: Protein-drug interactions monitored by time-resolved NMR in human cells (15’)April 21st, 2021.
10. CCP4 Virtual Study Weekend 2021. Invited SpeakerTitle: Protein-drug interactions monitored by time-resolved NMR in human cells (20’), January 5th – 8th, 2021.
11. Instruct-ULTRA General Assembly. Invited Speaker Title: An NMR bioreactor system for monitoring intracellular events in real time (10’), November 26th, 2020.
12. iNEXT-Discovery webinar series: What can iNEXT-Discovery do for us?Title: Real-time ligand binding in human cells monitored by bioreactor-assisted in-cell NMR (30’), November 23rd, 2020.
13. Webinar – Under 35 GIDRM Award, 5th ed.Title: In-cell NMR in human cells: from protein maturation to real-time ligand screening (30’), October 30th, 2020.
14. Biophysical Society virtual event: Magnetic Resonances Meet the Future of Biophysics. Invited SpeakerTitle: Intracellular protein-drug interactions monitored in real time by in-cell NMR (30’), September 25th, 2020.
15. Instruct-ERIC webinar series: structure meets functionTitle: Drug screening by in-cell NMR (20’), September 18th, 2020.
La sua ricerca si concentra sullo sviluppo e sull'applicazione di metodi di spettroscopia di risonanza magnetica nucleare in cellula (in-cell NMR) per studiare la struttura, la dinamica e le interazioni delle proteine a livello atomico all'interno di cellule vive. Ha contribuito a sviluppare un approccio innovativo basato sull'espressione di proteine marcate isotopicamente in cellule umane, che consente di studiare i processi di folding e maturazione delle proteine a livello atomico. Ha inoltre contribuito allo sviluppo di un bioreattore NMR in flusso in grado di preservare la vitalità delle cellule per diversi giorni, e alla sua applicazione allo studio di eventi intracellulari in tempo reale.
Attualmente si concentra sullo studio delle interazioni proteina-ligando all’interno della cellula, utilizzando approcci in-cell NMR sia per osservare le proteine che i ligandi. Sta inoltre sviluppando nuovi schemi di marcatura isotopica per le cellule umane, basati sull'incorporazione di amminoacidi fluorurati e sull'uso di precursori marcati di specifici amminoacidi.
La combinazione di queste metodologie consente di caratterizzare aspetti strutturali e meccanicistici di macromolecole biologiche nel loro ambiente cellulare, con grande rilevanza fisio-patologica.
Legenda
Enrico Luchinat was born in 1985. He graduated in 2007 with a B.Sc. in Chemistry cum laude and in 2009 with a M.Sc. in Chemistry of Biological Molecules cum laude at the University of Florence. In 2013, he obtained a Ph.D. in the International Doctorate of Mechanistic and Structural Systems Biology at the same University, under the supervision of Prof. Lucia Banci. He has been Postdoctoral fellow (2013-2019) at the Department of Experimental and Clinical Biochemical Sciences and Research fellow at NEUROFARBA Department (2020-2021). In 2022, he joined the Department of Agricultural and Food Sciences and Technologies at the Bologna University as RTDa Junior Assistant Professor, and in 2024 he joined the Chemistry Department of the University of Florence as Tenure-track Assistant Professor (RTDb).
He has co-authored more than 45 peer-reviewed publications and has been invited as speaker at more than 30 international conferences and virtual events. In 2020, he was awarded the GIDRM Under-35 Award for the development of innovative in-cell NMR approaches.
Research Unique Identifiers
Current position
12/2023 – today Senior Assistant Professor (tenure-track) Department of Chemistry “Ugo Schiff”, University of Florence, Italy
Previous positions
2021 – 2023 Junior Assistant Professor Department of Agri-Food Science and Technology, University of Bologna, Italy
2013 – 2021 Post-Doctoral Researcher Magnetic Resonance Centre – CERM, Department of Experimental and Clinical Biochemical Sciences (2013-2020) and Department of Experimental and Clinical Medicine Neurosciences, Psychology, Drug Research and Child Health (2020-2021), University of Florence, Italy
Grants and Fellowships
11/2014 – 02/2015 EMBO Short Term Fellowship.
European Molecular Biology Laboratory, Heidelberg, Germany. Funding: 8,313.10 €
10/2019 – 09/2021 Joint Research Award “Enabling user access to bioreactors for real-time in-cell NMR”, Instruct-ERIC pan-European research infrastructure in structural biology. Funding: 20,000 €
09/2023 – 09/2025 PRIN2022 “TiReD - Time-resolved magnetic resonance to investigate dynamic events in biological systems and biotransformations”, Italian Ministry of Research. Total funding: 208,000 €. Lead Unit: University of Florence; Partner Units: University of Bologna, University of Naples.
Awards
2020 Under 35 GIDRM Award. Gruppo Italiano Discussione Risonanze Magnetiche, 5th edition
Teaching Activities
AA 24-25 “Laboratorio di Chimica Inorganica I” (6 CFU), Master Degree in Chemistry, Università di Firenze. Italian; “Superstructures and Multi-Components Architectures in Life and Material Sciences” (2 CFU), Master Degree in Advanced Molecular Sciences, Università di Firenze. English.
AA 23-24 “Proteine e Loro Interazioni con Laboratorio” (1 CFU), Master Degree in Molecular Biotechnologies, Università di Firenze. Italian.
AA 22-23 “Chimica Strutturale dei Sistemi Biologici” (4 CFU), Master Degree in Food Science and Technology, DISTAL, Università di Bologna. Italian.
AA 21-22 “Chimica Strutturale dei Sistemi Biologici” (4 CFU), Master Degree in Food Science and Technology, DISTAL, Università di Bologna. Italian.
12/2022 “In-cell NMR”, within the “International Doctorate in Structural Biology” (2 CFU). English.
06/2019 “In-cell NMR”, within the “International Doctorate in Structural Biology” (2 CFU). English.
Tutoring activity
Co-tutor of 3 BSc and 1 MSc from 2021 to 2023, tutor of 1 MSc in 2024, co-supervisor of 2 PhD students (2023-2025) and supervisor of 1 PhD student (2025-2027).
Peer-reviewing activity
Organization of conferences and workshops
1. iNEXT-Discovery In-cell NMR Training Course, CERM, Sesto Fiorentino, Italy, 2023. Organizer
2. Instruct Practical School on Advanced Isotopic Labelling Methods for NMR, IBS – Grenoble, France, 2022. Co-organizer
3. Instruct Training Course: In-cell NMR Analysis of Biomolecular Structure and Function, CERM, Sesto Fiorentino, Italy, 2013. Organizing committee
4. 12th Chianti/Instruct Workshop on BioNMR - Electron and Nuclear Relaxation for Structural Biology, Montecatini Terme, Italy, 2012. Organizing committee
Bibliometric Indexes
h-index: 25 (Scopus), 26 (Google Scholar)Citations: 1749 (Scopus), 2321 (Google Scholar)Publications: 49Book Chapters: 4
Scientific Publications
11. Luchinat, E.*, Barbieri, L., Cremonini, M., Pennestri, M., Nocentini, A., Supuran, C.T., Banci, L.* Determination of intracellular protein–ligand binding affinity by competition binding in-cell NMR. Acta Crystallographica Section D: Structural Biology, 77(10) (2021).IF: 7.652
28. Barbieri, L., Luchinat, E., & Banci, L. Intracellular metal binding and redox behavior of human DJ-1. JBIC Journal of Biological Inorganic Chemistry, 23(1), 61-69 (2018).IF: 2.952
32. Luchinat, E., & Banci, L. In-cell NMR: a topical review. IUCrJ, 4(2), 108-118 (2017).IF: 6.544
34. Barbieri, L., Luchinat, E., & Banci, L. Characterization of proteins by in-cell NMR spectroscopy in cultured mammalian cells. Nature protocols, 11(6), 1101-1111 (2016).IF: 12.423
Books/Chapters
Presentations at Conferences as Invited Speaker
1. EUROMAR 2024 - The 20th European Magnetic Resonance Congress, Bilbao, Spain, June 30th - July 4th 2024.
Invited Speaker. Title: Multi-target ligand binding assay in human cells by real-time in-cell 19F NMR
2. Final iNEXT-Discovery Consortium Meeting and 4th Symposium on Recent Advances in Cryo-EM, Brno, Czech Republic, June 10th-13th 2024.Invited Speaker. Title: Detecting protein-drug interactions in human cells by real-time 19F NMR
8. ICGEB course “NMR for combatting diseases: from cancer to SARS-CoV-2”, CERM, Sesto Fiorentino, Italy, March 27th – 31st 2023.Invited Speaker. Title: In-cell NMR for real-time studies of protein-ligand interactions in human cells
15. International Practical School on Advanced Isotopic Labelling Methods for NMR, IBS, Grenoble, France, 29th March – April 5th 2019.Instructor. Title: Practical aspects of in cell NMR spectroscopy in mammalian cells
27. The 26th Annual Symposium of The Protein Society, San Diego, CA, U.S.A., August 5th-8nd, 2012.Invited speaker. Title: Protein maturation steps monitored with atomic resolution in living cells by NMR
Invited Speaker at Webinar and Virtual Conferences
2. Seminar – Forschungszentrum JülichTitle: In-cell NMR in human cells: from protein maturation to real-time ligand screening (50’)March 8th 2022
3. Molecular Biosciences PhD program ITQB-Nova, Lisbon, Portugal. TeacherTitle: In-cell NMR in human cells: from protein maturation to real-time ligand screening (50’)February 24th 2022
4. TIMB3 Training School - Fundamentals of magnetic resonance spectroscopies and metal trafficking. LecturerTitle: In-cell NMR in human cells: from protein maturation to real-time ligand screening (50’)October 22nd 2021
5. Seminar – Oregon State UniversityTitle: In-cell NMR in human cells: from protein maturation to real-time ligand screening (50’)October 6th 2021
6. EUROMAR 2021 Conference. Invited SpeakerTitle: Protein-drug interactions monitored by time-resolved NMR in human cells (20')July 5th – 8th, 2021.
7. Message From The Cell - A workshop on biophysical studies for structural biology inside cells. Invited SpeakerTitle: Protein-drug interactions monitored by time-resolved NMR in human cells (30')June 24th – 25th, 2021.
11. Instruct-ULTRA General Assembly. Invited SpeakerTitle: An NMR bioreactor system for monitoring intracellular events in real time (10’), November 26th, 2020.
His research focuses on the development and application of in-cell NMR spectroscopic tools to study protein structure, dynamics and interactions at the atomic level inside intact, living cells. He contributed to pioneer an approach based on the direct expression of isotope-labeled proteins in cultured human cells, which allows studying protein folding and maturation processes at the residue-level. He contributed to develop a modular flow NMR bioreactor capable of preserving cell viability for several days, and applied it to monitor intracellular events in real time.
He is currently focusing on the study of intracellular protein-ligand interactions, both by protein- and ligand-observe in-cell NMR approaches. He is also developing novel labeling schemes for human cells, based on the incorporation of fluorinated amino acids and the use of labelled amino acid precursors.
Combined together, these tools provide unique structural and mechanistic insights in a highly physiological cellular setting.
