ATTIVITA’ DI RICERCA
15/12/2022-ad oggi
Ricercatore presso l’Università degli Studi di Firenze impegnata nel progetto “Identification and targeting with RNA-based approaches of the metabolic-dependent and-independent networks in tumor microenvironment” il cui responsabile scientifico è la prof. Elisa Giannoni.
2020-2022
3 anni di borsa di studio finanziata da FIRC-AIRC per lo svolgimento del progetto dal titolo “Metabolic adaptations driving epigenetics of 5 Fluorouracil-resistant colon cancer: the role of one-carbon metabolism” press il dip.to di Sienze Biomediche, Sperimentali e Cliniche (Università degli Studi di Firenze) sotto la supervisione della prof. Paola Chiarugi.
2019
6 mesi come visiting PhD student presso Laboratory of Cellular Metabolism and Metabolic Regulation, VIB-KU Leuven Center for Cancer Biology, Leuven (Belgium) sotto la supervision della Prof. Sarah-Maria Fendt.
2018
2 mesi come PhD student at CRUK, Wolfson Wohl Cancer Research Centre, University of Glasgow (UK) sotto la supervision del prof. Oliver Maddocks.
2016-2019
Dottorato di ricercar in Biochimica e Biologia molecolare, Università di Siena, svolgendo l’attività di ricerca presso il dip.to di Scienze Biomediche, Sperimentali e Cliniche (Università degli studi di Firenze). Il progetto di ricerca condotto era volto allo studio degli adattamenti metabolici legati all’acquisizione della resistenza al 5-FU nel colon carcinoma.
2016
6 mesi di tirocinio (Erasmus+ Traineeship) presso University of Louvain (UCL), Medical School, Pole of Pharmacology and Therapeutics-Brussels (Belgium) sotto la supervisione del Pierre Sonveaux.
2013-2014
6 mesi di tirocinio presso dip.to di Scienze Biomediche, Sperimentali e Cliniche (Università degli studi di Firenze) sotto la supervisione del prof. Paolo Paoli.
PUBBLICAZIONI
-“Sex‐related changes in lactate dehydrogenase A expression differently impact the immune response in melanoma” Iozzo M., Comito G., Ippolito L., Sandrini G., Pardella E., Pranzini E., […], Giannoni E. .FEBS Journal (2024)
-“Limiting serine availability during tumor progression promotes muscle wasting in cancer cachexia” Pranzini E., Muccillo L., Nesi I., […], Paoli P. Cell Death Discovery (2024)
-“Lactate supports cell-autonomous ECM production to sustain metastatic behavior in prostate cancer”Ippolito, L., Duatti A., Iozzo M., Comito G., Pardella E., Lorito N., Bacci M., Pranzini E., […], Chiarugi P. EMBO Reports (2024)
-“Adapt and shape: metabolic features within the metastatic niche” Pranzini E., Ippolito, L., Pardella, E., Giannoni, E., Chiarugi, P. Trends in Endocrinology and Metabolism (2024)
-“Metabolic challengers selecting tumor-persistent cells” Mancini, C., Lori, G., Pranzini, E., Taddei, M.L. Trends in Endocrinology and Metabolism (2024)
-“Chemoenzymatic Synthesis of Glycopeptides to Explore the Role of Mucin 1 Glycosylation in Cell Adhesion” Bello, C., Pranzini, E., Papini A.M.ChemBioChem (2023);
-“A New Antidiabetic Agent Showing Short- and Long-Term Effects Due to Peroxisome Proliferator-Activated Receptor Alpha/Gamma Dual Agonism and Mitochondrial Pyruvate Carrier Inhibition
Laghezza, A.” Cerchia C., Genovese M., Leuci R., Pranzini E.,
Loiodice, F. Journal of Medicinal Chemistry (2023);
-“Evidence of Insulin-Sensitizing and Mimetic Activity of the Sesquiterpene Quinone Avarone, a Protein Tyrosine Phosphatase 1B and Aldose Reductase Dual Targeting Agent from the Marine Sponge Dysidea avara” Casertano M., Genovese M., Santi, A., Pranzini E., Paoli, P. Pharmaceutics (2023);
-“Targeting of tumor cells by custom antigen transfer: a novel approach for immunotherapy of cancer”Nesi I., Della Bella C., Taddei M.L., Santi A., Pranzini E., […], Caselli A., Cirri P. Frontiers in Oncology (2023);
-“Evidence of Insulin-Sensitizing and Mimetic Activity of the Sesquiterpene Quinone Avarone, a Protein Tyrosine Phosphatase 1B and Aldose Reductase Dual Targeting Agent from the Marine Sponge Dysidea avara” Casertano M., Genovese M., Santi A., Pranzini E., […], Menna M., Paoli P. Pharmaceutics (2023);
-“Therapy-Induced Stromal Senescence Promoting Aggressiveness of Prostate and Ovarian Cancer” Pardella E., Pranzini E., Nesi, I., […], Magherini, F., Taddei, M.L. Cells (2022);
-“Transcriptomic Analysis of Colorectal Cancer Cells Treated with Oil Production Waste Products (OPWPs) Reveals Enrichment of Pathways of Mitochondrial Functionality” Leo, M., Muccillo, L., Pranzini, E., [...],Taddei, M.L., Sabatino, L; Cells (2022);
-“SHMT2-mediated mitochondrial serine metabolism drives 5-FU resistance by fueling nucleotide biosynthesis” Pranzini E., Pardella E., Muccillo L., […], Chiarugi P., Maddocks O.D.K., Paoli P., Taddei M.L.; Cell Reports (2022);
-“MitoQ Prevents Human Breast Cancer Recurrence and Lung Metastasis in Mice” Capeloa T., Krzystyniak J., Canas Rodriguez A., Payen V.L., Zampieri L.X., Pranzini E., […], Porporato P.E., Sonveaux P.; Cancers (2022);
-“MitoQ Inhibits Human Breast Cancer Cell Migration, Invasion and Clonogenicity” Capeloa T., Krzystyniak J., d’Hose D., Canas Rodriguez A., Payen V.L., Zampieri L.X., A Van de Velde J., Benyahia Z., Pranzini E., […], Porporato P.E., Sonveaux P.; Cancers (2022);
-“Metabolic Features of Tumor Dormancy: Possible Therapeutic Strategies” Pranzini E., Raugei G., Taddei M.L.; Cancers (2022);
-“The miR-27a/FOXJ3 Axis Dysregulates Mitochondrial Homeostasis in Colorectal Cancer Cells” Barisciano G., Leo M., Muccillo L., Pranzini E., Parri M., Colantuoni V., Taddei M.L., Sabatino L.; Cancers (2022);
-“Claisened hexafluoro inhibits metastatic spreading of amoeboid melanoma cells” Leo A., Pranzini E., Pietrovito L., Pardella E., Parri M., Cirri P., Bruno G., Calvani M., Peppicelli S., Torre E., Sasaki M., Yang L., Zhu L., Chiarugi P., Raugei G., Arbiser J.L., Taddei M.L.; Cancers (2022);
-“Metabolic Reprogramming in Anticancer Drug Resistance: A Focus on Amino Acids” Pranzini E., Pardella E., Paoli P., Fendt S.M., Taddei M.L.; Trends in Cancer (2021);
-Mitochondrial oxidative metabolism contributes to a cancer stem cell phenotype in cholangiocarcinoma” Raggi C., Taddei M.L., Sacco E., Navari N., Correnti M., Piombanti B., Pastore M., Campani C., Pranzini E., Iorio J., Lori G., Lottini T., Peano C., Cibella J., Lewinska M., Andersen J.B., Tommaso L., Luca Vigano, Di Maira G., Madiai S., Ramazzotti M., Orlandi I., Arcangeli A., Chiarugi P., Marra F.; Journal of Hepatology (2021);
-“In Vivo Evidence for Serine Biosynthesis-Defined Sensitivity of Lung Metastasis, but Not of Primary Breast Tumors, to mTORC1 Inhibition” Rinaldi G., Pranzini E., Van Elsen J., Broekaert D., Funk C.M., Planque M., Doglioni G., Altea-Manzano P., Rossi M., Geldhof V., Thing Teoh S., Ross C., Hunter K.W., Lunt S.Y., Grünewald T.G.P., Fendt S.M.; Molecular Cell (2021);
-“Role of tyrosine phosphorylation in modulating cancer cell metabolism.” Taddei M.L., Pardella E., Pranzini E., Raugei G., Paoli P.; Biochimica et Biophysica Acta (BBA) - Reviews on Cancer (2020);
-“Oncogenic Tyrosine Phosphatases: Novel Therapeutic Targets for Melanoma Treatment.” Pardella E.†, Pranzini E.†, Leo A., Taddei M.L., Paoli P., Raugei G.; Cancers (2020); (†authors that equally contributed to the work).
-“Stable Isotopes for Tracing Mammalian-Cell Metabolism in vivo.” Fernández-García J., Altea-Manzano P., Pranzini E., Fendt S.M.; Trends in Biochemical Sciences (2020);
-“miR-210-3p mediates metabolic adaptation and sustains DNA damage repair of resistant colon cancer cells to treatment with 5-fluorouracil.” Pranzini E., Leo A., Rapizzi E., Ramazzotti M., Magherini F., Giovannelli L., Caselli A., Cirri P., Taddei ML., Paoli P.; Molecular Carcinogenesis (2019);
-“Multiwalled Carbon Nanotubes for combination therapy: a biodistribution and efficacy pilot study.” Biagiotti G., Pisaneschi F., Gammon S.T., Machetti F., Ligi M.C., Giambastiani G., Tuci G., Powell E., Piwnica-Worms H., Pranzini E., Paoli P., Cicchi S., Piwnica-Worms D.; Journal of Materials Chemistry B (2019);
-"LMW-PTP modulates glucose metabolism in cancer cells.” Lori G., Gamberi T., Paoli P., Caselli A., Pranzini E., Marzocchini R., Modesti A., Raugei G.; Biochimica et Biophysica Acta-General Subjects (2018);
-"Metformin salts with oxidized multiwalled carbon nanotubes: In vitro biological activity and inhibition of CNT internalization.” Biagiotti G., Ligi M.C., Fedeli S., Pranzini E., Gamberi T., Cicchi S., Paoli P.; Journal of Drug Delivery Science and Technology (2018);
-"Morin-dependent inhibition of low molecular weight protein tyrosine phosphatase (LMW-PTP) restores sensitivity to apoptosis during colon carcinogenesis: studies in vitro and in vivo, in an Apc-driven model of colon cancer." Lori G., Paoli P., Femia A.P., Pranzini E., Caselli A., Tortora K., Romagnoli A., Raugei G., Caderni G.; Molecular carcinogenesis (2018).
EDUCAZIONE
2021-2022
Master in Scientific Communication, Università di Parma.
Voto finale: 110/110 cum laude.
Data della discussione: 12/10/2022.
2016 -2020
Dottorato in Biochemistry and Molecular Biology, Università di Siena, tesi dal titotlo“Metabolic reprogramming of colorectal cancer cells resistant to 5-FU”.
Supervisor: Prof. Paolo Paoli (University of Florence)
Data della discussione: 24/02/2020.
2014-2016
Laurea Magistrale in Biologia applicate alla Biomedicina, Università di Pisa, tesi dal titolo “mtROS scavenging reduces cisplatin-induced cell migration in triple negative breast cancer”.
Supervisors: Prof. Maria Grazia Tozzi (University of Pisa), Dr. Paolo Porporato (Université catholique de Louvain).
Data della discussione: 24/10/2016.
2011-2014
Laurea Triennale in Biologia, Università di Florence, tesi dal ttolo “Effects of DASA-58 on metabolism and proliferation of colon carcinoma cells".
Supervisor: Prof. Paolo Paoli.
Voto finale: 110/110 cum laude, with encomium.
Data della discussione: 23/07/2014.
2010
Diploma liceo Scientifico,Liceo “P. Gobetti”, Bagno a Ripoli (FI).
Voto finale: 100/100.
Legenda
RESEARCH ACTIVITY
15/12/2022-to date
Research Fellow (RTD-A) at the University of Florence working on the project “Identification and targeting with RNA-based approaches of the metabolic-dependent and-independent networks in tumor microenvironment” under the supervision of Prof. Elisa Giannoni.
Three years Fellowship awarded by FIRC-AIRC to carry out the research project “Metabolic adaptations driving epigenetics of 5 Fluorouracil-resistant colon cancer: the role of one-carbon metabolism” at the Department of “Biomedical Sciences, experimental and clinical” (University of Florence) under the supervision of Prof. Paola Chiarugi.
6 months as visiting PhD student at the Laboratory of Cellular Metabolism and Metabolic Regulation, VIB-KU Leuven Center for Cancer Biology, Leuven (Belgium) under the supervisor of Prof. Sarah-Maria Fendt.
2 months as vising PhD student at CRUK, Wolfson Wohl Cancer Research Centre, University of Glasgow (UK) under the supervision of prof. Oliver Maddocks.
PhD in Biochemistry and Molecular Biology, University of Siena. Research activity held at the Department of “Biomedical Sciences, experimental and clinical” (University of Florence) investigating the role of metabolic reprogramming of drug-resistant colon cancer cells.
6 months of internship (Erasmus+ Traineeship) at the University of Louvain (UCL), Medical School, Pole of Pharmacology and Therapeutics-Brussels (Belgium), under the supervision of prof. Pierre Sonveaux.
6 months of internship at the Department of “Biomedical Sciences, experimental and clinical” (University of Florence) under the supervision of Prof. Paolo Paoli.
PUBLICATIONS
EDUCATION
Master in Scientific Communication, University of Parma.
Final grade: 110/110 cum laude.
Date of final dissertation: 12/10/2022.
PhD in Biochemistry and Molecular Biology, University of Siena, discussing a thesis titled “Metabolic reprogramming of colorectal cancer cells resistant to 5-FU”.
Date of final dissertation: 24/02/2020.
Master’s Degree in Biology applied to Biomedicine, University of Pisa, discussing an experimental thesis titled “mtROS scavenging reduces cisplatin-induced cell migration in triple negative breast cancer”.
Supervisors: Prof. Maria Grazia Tozzi (University of Pisa) and Dr. Paolo Porporato (Université catholique de Louvain).
Date of final dissertation: 24/10/2016.
Bachelor's Degree in Biology, University of Florence, discussing an experimental thesis titled “Effects of DASA-58 on metabolism and proliferation of colon carcinoma cells".
Final grade: 110/110 cum laude, with encomium.
Date of final dissertation: 23/07/2014.
Scientific High School Diploma, “P. Gobetti” high school, Bagno a Ripoli (FI).
Final mark: 100/100.
